This is module 7 of the NeoPeptidos Professional Certification in Peptide Science. It has 4 lessons; each one can be read on its own and all of them are part of the syllabus assessed at the end of the module.
Lesson 1. HPLC: the measure of purity
An experimental result is only worth as much as the characterization of the material that produced it. Two questions structure quality control: is it what it claims to be? (identity) and how pure is it? (purity).
Read the lesson →Lesson 2. Mass spectrometry and identity
Purity without identity is not enough: you could have 99% of the wrong molecule. Mass spectrometry (MS) confirms identity by measuring molecular weight with high accuracy.
Read the lesson →Lesson 3. Peptide content, water, counterion and reading the COA
Beyond identity and purity, there are parameters that describe the actual composition of the powder in the vial:
Read the lesson →Lesson 4. Related impurities and counterions
A purity of 99% means up to 1% of the chromatogram area corresponds to something else. Knowing what that «something else» usually is lets you read a COA with judgment and understand why two batches with the same purity may not be equivalent.
Read the lesson →Module quiz
When you finish the lessons, take the quiz on the course page. Your progress is saved to your account.
Go to the quiz →Educational content for scientific purposes. It distinguishes preclinical from clinical evidence and is not medical advice, diagnosis or a recommendation for use. NeoPeptidos products are sold labelled for research use only (RUO).