Reconstitution guide
Enter your vial's details and instantly get the concentration, volume and syringe units to draw. For research reference only.
Enter your vial's details to calculate concentration and draw volumes
Draw 10 units of solution into your U-100 insulin syringe to get approximately 250 mcg of peptide. Each unit = 0.01 mL.
Follow these steps to ensure proper reconstitution of your compounds in the laboratory.
Gather the lyophilized peptide vial, the bacteriostatic water (included in your order), sterile insulin syringes, latex or nitrile gloves and 70% isopropyl alcohol wipes. Work on a clean, disinfected surface.
Use the calculator above to determine how much bacteriostatic water you need. Choose a volume that results in an easy-to-calculate concentration. For example, for a 5 mg vial, adding 2 mL gives a concentration of 2,500 mcg/mL.
Clean the rubber stopper of the peptide vial and of the bacteriostatic water vial with an alcohol wipe. Let it air dry for 30 seconds before inserting any needle. Never blow on the stopper.
With a sterile syringe, draw the calculated amount of bacteriostatic water from the vial. Hold the water vial upside down to make drawing easier and avoid air bubbles.
Tilt the peptide vial slightly and direct the stream of water toward the inner wall of the vial — not directly onto the powder. Inject slowly to avoid foam. Never shake the vial hard, as it can degrade the peptide.
Roll the vial between your palms with gentle circular movements. Most peptides dissolve in 30–60 seconds. The liquid should be completely clear or slightly opalescent. If visible particles remain, keep rolling (never shake).
Your vial is already labeled, so just add the reconstitution date. Refrigerate immediately between 2 and 8 °C, protected from light.
It is recommended to use it within the following 60 days; after that the peptide may lose bioavailability.
Differences between the most common units of measurement in peptide reconstitution.
1 mg = 1,000 mcg. The peptide is weighed in milligrams (mg), but research doses are usually expressed in micrograms (mcg). Always check the unit before calculating.
All U-100 insulin syringes have the same scale: 1 unit = 0.01 mL. What changes is the maximum capacity — 100u (1.0 mL), 50u (0.5 mL) or 30u (0.3 mL). For small doses, a 30u syringe allows greater visual precision.
mcg/mL = micrograms per milliliter. It is the amount of peptide dissolved in each mL of solution. Example: 2,500 mcg/mL means each mL contains 2,500 mcg of peptide.
The volume (mL) you draw with the syringe depends on the concentration of your solution. The higher the concentration, the smaller the volume for the same peptide dose. The calculator does this conversion automatically.
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