A factory-sealed vial is a closed system. Every time its stopper is punctured a possible route of contamination opens, and aseptic technique consists of keeping that risk to a minimum at every step.
- Work on a clean surface with washed hands.
- Disinfect the rubber stopper with 70% isopropyl alcohol and let it dry before puncturing it.
- Always use new needles and syringes, without touching the needle or the disinfected stopper.
- Do not return liquid that has left the vial or share equipment between vials.
The benzyl alcohol in bacteriostatic water adds a margin against small contaminations in vials used repeatedly, but it does not replace these practices.
In research, microbial or endotoxin contamination alters results in ways that are hard to detect. Aseptic technique protects both the material and the validity of the experiment.
Related terms
Learn it in depth at the Peptide University
- Reconstitution and aseptic technique · Module 9
- Technical fundamentals of subcutaneous administration · Module 8
- The lyophilized form and its diluents · Module 8
- Practical calculation cases and common errors · Module 9
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Definition for educational and scientific purposes. It is not medical advice or a recommendation for use. NeoPeptidos products are sold labelled for research use only (RUO).